Riccardo D'Incà, Roberto Mattioli, Martina Tomasella, Raffaela Tavazza, Alberto Macone, Alessio Incocciati, Damiano Martignago, Fabio Polticelli, Ilaria Fraudentali, Alessandra Cona, Riccardo Angelini, Mario Tavazza, Andrea Nardini, Paraskevi Tavladoraki
Polyamines are involved in several plant physiological processes. In Arabidopsis thaliana, five FAD-dependent polyamine oxidases (AtPAO1 to AtPAO5) contribute to polyamine homeostasis. AtPAO5 catalyzes the back-conversion of thermospermine (T-Spm) to spermidine and plays a role in plant development, xylem differentiation, and abiotic stress tolerance. In the present study, to verify whether T-Spm metabolism can be exploited as a new route to improve stress tolerance in crops and to investigate the underlying mechanisms, tomato (Solanum lycopersicum) AtPAO5 homologs were identified (SlPAO2, SlPAO3, and SlPAO4) and CRISPR/Cas9-mediated loss-of-function slpao3 mutants were obtained...
May 18, 2024: Plant Journal